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Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00359988" target="_blank" >RIV/61388963:_____/11:00359988 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1016/j.ab.2011.01.048" target="_blank" >http://dx.doi.org/10.1016/j.ab.2011.01.048</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.ab.2011.01.048" target="_blank" >10.1016/j.ab.2011.01.048</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing

  • Original language description

    Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Analytical Biochemistry

  • ISSN

    0003-2697

  • e-ISSN

  • Volume of the periodical

    413

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    8-15

  • UT code for WoS article

    000289607900002

  • EID of the result in the Scopus database