Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00359988" target="_blank" >RIV/61388963:_____/11:00359988 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ab.2011.01.048" target="_blank" >http://dx.doi.org/10.1016/j.ab.2011.01.048</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ab.2011.01.048" target="_blank" >10.1016/j.ab.2011.01.048</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of glycated hemoglobin A1c by capillary electrophoresis and capillary isoelectric focusing
Original language description
Two capillary electrophoretic methods were developed and evaluated for measurement of glycated hemoglobin HbA1c. Firstly, a capillary zone electrophoresis analysis is performed with a sodium tetraborate buffer of pH 9.3 as background electrolyte in a neutrally coated capillary. HbA1c is separated from HbA0 due to specific interactions of borate anions with the cis-diol pattern in the saccharide moiety of glycohemoglobin. Secondly, a capillary isoelectric focusing method, which exploits a difference in pI values of HbA0 and HbA1c, is performed with Servalyt pH 6-8 or alternatively with Biolyte pH 6-8 carrier ampholytes spiked with a narrow pH cut of pH 7.2 prepared by preparative fractionation of Servalyt pH 4-9 carrier ampholytes.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Biochemistry
ISSN
0003-2697
e-ISSN
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Volume of the periodical
413
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
8-15
UT code for WoS article
000289607900002
EID of the result in the Scopus database
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