All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00364221" target="_blank" >RIV/61388963:_____/11:00364221 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1021/bi2006916" target="_blank" >http://dx.doi.org/10.1021/bi2006916</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/bi2006916" target="_blank" >10.1021/bi2006916</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    B Family DNA Polymerases Asymmetrically Recognize Pyrimidines and Purines

  • Original language description

    We utilized a series of modified pyrimidine analogues to determine the mechanism used by human DNA polymerase ? and herpes simplex virus I DNA polymerase to polymerize pyrimidine dNTPs. Removing O2 of a pyrimidine dNTP vastly decreased incorporation by these enzymes and also compromised fidelity in the case of C analogues, while removing O2 from the templating base had more modest effects. Removing the Watson-Crick hydrogen bonding groups greatly impaired polymerization. The Watson-Crick hydrogen bonding plays an important role in enhancing correct dNTP polymerization, but are not essential for preventing misincorporation. These studies also indicate that DNA polymerases recognize bases extremely asymmetrically, both in terms of whether they are a purine or pyrimidine and whether they are in the template or are the incoming dNTP. The mechanistic implications of these results regarding how polymerases discriminate between right and wrong dNTPs are discussed.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CC - Organic chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biochemistry

  • ISSN

    0006-2960

  • e-ISSN

  • Volume of the periodical

    50

  • Issue of the periodical within the volume

    33

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    7243-7250

  • UT code for WoS article

    000294076100018

  • EID of the result in the Scopus database