Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00369816" target="_blank" >RIV/61388963:_____/11:00369816 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/pro.744" target="_blank" >http://dx.doi.org/10.1002/pro.744</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/pro.744" target="_blank" >10.1002/pro.744</a>
Alternative languages
Result language
angličtina
Original language name
Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall
Original language description
We found that Sapp1p of C. parapsilosis displays enzyme activity prior to secretion, and therefore, it is probably fully folded within the upper layer of the cell wall. The positioning of cell surface-associated Sapp1p was detected by cell wall protein labeling using biotinylation agents, extraction of cell wall proteins by beta-mercaptoethanol, immunochemical detection, and mass spectrometry analysis. The retaining of Sapp1p in the cell wall is not a totally random process and pathogenic yeasts might use this cell-associated proteinase activity to enhance degradation of appropriate substrates.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Protein Science
ISSN
0961-8368
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
2004-2012
UT code for WoS article
000297462400006
EID of the result in the Scopus database
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