Raman optical activity study on insulin amyloid- and prefibril intermediate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00377069" target="_blank" >RIV/61388963:_____/12:00377069 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/chir.21029" target="_blank" >http://dx.doi.org/10.1002/chir.21029</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chir.21029" target="_blank" >10.1002/chir.21029</a>

Result language

angličtina

Original language name

Raman optical activity study on insulin amyloid- and prefibril intermediate

Original language description

The amyloid fibril of bovine insulin and its renaturing intermediates were studied by using Raman optical activity (ROA). In the spectrum of the amyloid, the sharp +/- ROA couplet of amide I band characteristic of the beta-sheet-rich proteins was observed, together with a sharp peak at 1271 cm(-1) characteristic of a turn structure. The shoulder ROA peak of the native insulin at similar to 1340 cm(-1), which was assigned to the hydrated alpha-helix, was not observed in the amyloid, suggesting that the hydrated alpha-helix was converted to the parallel beta-sheet structure in the amyloid. Recovery of the amyloid to the native state was also monitored by ROA. The intermediate states showed distinct features from the amyloid or native ones. The intermediates did not show a characteristic ROA peak of the poly(L-proline) II helix at similar to 1318 cm(-1). The hydrated alpha-helix ROA peak was not recovered in the intermediate states. In a process of the amyloid formation, at first the hydr

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chirality

ISSN

0899-0042

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

97-103

UT code for WoS article

000298873000001

EID of the result in the Scopus database

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