Use of Four Next-Generation Sequencing Platforms to Determine HIV-1 Coreceptor Tropism
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00383618" target="_blank" >RIV/61388963:_____/12:00383618 - isvavai.cz</a>
Result on the web
<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0049602" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0049602</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0049602" target="_blank" >10.1371/journal.pone.0049602</a>
Alternative languages
Result language
angličtina
Original language name
Use of Four Next-Generation Sequencing Platforms to Determine HIV-1 Coreceptor Tropism
Original language description
HIV-1 coreceptor tropism assays are required to rule out the presence of CXCR4-tropic (non-R5) viruses prior treatment with CCR5 antagonists. Phenotypic (e.g., Trofile (TM), Monogram Biosciences) and genotypic (e.g., population sequencing linked to bioinformatic algorithms) assays are the most widely used. Although several next-generation sequencing (NGS) platforms are available, to date all published deep sequencing HIV-1 tropism studies have used the 454 (TM) Life Sciences/Roche platform. In this study, HIV-1 co-receptor usage was predicted for twelve patients scheduled to start a maraviroc-based antiretroviral regimen. The V3 region of the HIV-1 env gene was sequenced using four NGS platforms: 454 (TM), PacBio (R) RS (Pacific Biosciences), Illumina(R), and Ion Torrent (TM) (Life Technologies). Cross-platform variation was evaluated, including number of reads, read length and error rates. HIV-1 tropism was inferred using Geno2Pheno, Web PSSM, and the 11/24/25 rule and compared with
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LK11207" target="_blank" >LK11207: The Role of HIV Fitness on Disease Progression in the Absence of Antiretroviral Treatment</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
"e49602/1"-"e49602/17"
UT code for WoS article
000311151900171
EID of the result in the Scopus database
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