Atomic Scissors: A New Method of Tracking the 5-Bromo-2-Deoxyuridine-Labeled DNA In Situ

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00386203" target="_blank" >RIV/61388963:_____/12:00386203 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00386203 RIV/68378050:_____/12:00386203

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0052584" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0052584</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0052584" target="_blank" >10.1371/journal.pone.0052584</a>

Result language

angličtina

Original language name

Atomic Scissors: A New Method of Tracking the 5-Bromo-2-Deoxyuridine-Labeled DNA In Situ

Original language description

A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I) in the presence of oxygen, which leads to the abstraction of hydrogen atom from deoxyribose culminating in the elimination of the nucleobase, scission of the nucleic-acid strand and formation of frequent gaps. The gaps allow the reaction of the antibodies with the commonly used markers of replication (e.g. 5-bromo-2-deoxyuridine), which are otherwise masked. The method developed makes it possible to detect nuclear and mitochondrial DNA replication efficiently. In most cases, it does not inhibit effective protein detections and in addition enables simultaneous localization of newly-synthesized RNA. The alternative presently-used methods result in protein denaturation and/or extensive DNA cleavage followed by the DNA-bound proteins peeling off.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

—

Project

<a href="/en/project/KAN200520801" target="_blank" >KAN200520801: Targeted expression and transport of bioactive molecules</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

—

UT code for WoS article

000313618800107

EID of the result in the Scopus database

—