A Fraction of MCM 2 Proteins Remain Associated with Replication Foci During a Major Part of S Phase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10481" target="_blank" >RIV/00216208:11110/11:10481 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/11:00002949

Result on the web

<a href="http://fb.cuni.cz/volume-57-2011-no-1#articFB2011A0002" target="_blank" >http://fb.cuni.cz/volume-57-2011-no-1#articFB2011A0002</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A Fraction of MCM 2 Proteins Remain Associated with Replication Foci During a Major Part of S Phase

Original language description

The essential role of MCM 2-7 proteins in the initiation of DNA replication in all eukaryotes is well known. Their role in replication elongation is supported by numerous studies, but there is still a knowledge gap in this respect. Even though biochemical studies have established an association of MCM proteins with replication forks, previous immunofluorescence studies in mammalian cells have suggested that MCM 2-7 proteins are displaced after replication initiation from sites of DNA replication. Therefore, we used a robust statistical method to more precisely analyse immunofluorescence localization of MCM 2 proteins with respect to the DNA replication foci. We show that despite the predominantly different localization of MCM 2 and replication signals,there is still a small but significant fraction of MCM 2 proteins that co-localize with DNA replication foci during most of S phase. The fluorescence localization of the MCM 2 proteins and DNA replication may thus reflect an active funct

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

<a href="/en/project/LC535" target="_blank" >LC535: Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica (Praha)

ISSN

0015-5500

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

3-11

UT code for WoS article

000288623700002

EID of the result in the Scopus database

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