CRL4DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F25%3A00641301" target="_blank" >RIV/68081707:_____/25:00641301 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00641301 RIV/00216224:14310/25:00142586 RIV/00216208:11310/25:10515609
Result on the web
<a href="https://www.nature.com/articles/s41467-025-64258-5" target="_blank" >https://www.nature.com/articles/s41467-025-64258-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-025-64258-5" target="_blank" >10.1038/s41467-025-64258-5</a>

Result language
angličtina
Original language name
CRL4DCAF12 regulation of MCMBP ensures optimal licensing of DNA replication
Original language description
The minichromosome maintenance (MCM2-7) protein complexes are central drivers of genome duplication. Distinct protein pools, parental and nascent MCMs, and their precise equilibrium are essential to sustain error-free DNA replication. However, the mechanism responsible for generating these pools and maintaining their equilibrium remains largely unexplored. Here, we identified CRL4DCAF12 as a factor controlling the assembly of nascent MCM complexes. During MCM biogenesis, MCMBP facilitates the assembly and transport of newly synthesized MCM3-7 subcomplexes into the nucleus. Once in the nucleus, the MCM2 subunit must be incorporated into the MCM3-7 subcomplex, while MCMBP needs to be removed. CRL4DCAF12 facilitates the degradation of MCMBP and thereby regulates the assembly of MCM2-7 complexes. The absence of CRL4DCAF12 adversely affects the level of chromatin-bound nascent MCMs, resulting in accelerated replication forks and replication stress. Collectively, our findings uncovered the molecular mechanism underlying nascent MCM production essential to counteract genome instability.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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