Crystallization of the Effector-Binding Domain of Repressor DeoR from Bacillus subtilis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00392314" target="_blank" >RIV/61388963:_____/13:00392314 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/13:00392314
Result on the web
<a href="http://dx.doi.org/10.1021/cg301551v" target="_blank" >http://dx.doi.org/10.1021/cg301551v</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/cg301551v" target="_blank" >10.1021/cg301551v</a>
Alternative languages
Result language
angličtina
Original language name
Crystallization of the Effector-Binding Domain of Repressor DeoR from Bacillus subtilis
Original language description
The DeoR repressor of Bacillus subtilis negatively regulates the expression of the enzymes needed for the metabolism of deoxyribonucleosides and deoxyribose. To gain structural information on the regulation of DeoR function by a small molecular ligand, we prepared crystals of the C-terminal domain of DeoR in its free form and in complex with the effector molecule deoxyribose-5-phosphate. To develop the optimal procedure for crystallization, we have employed the thermofluor assay as a tool for the optimization of protein crystallizability. The monocrystals of three crystal forms were used to collect complete sets of diffraction data at a synchrotron radiation source and will be used to determine the DeoR crystal structure.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Crystal Growth & Design
ISSN
1528-7483
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
844-848
UT code for WoS article
000314795300054
EID of the result in the Scopus database
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