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Towards crystal structures of antibodies and transcription factors

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00398525" target="_blank" >RIV/61388963:_____/13:00398525 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378050:_____/13:00398525

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Towards crystal structures of antibodies and transcription factors

  • Original language description

    Protein three-dimensional structure determination by X-ray crystallography is important for understanding protein function. The protein crystallization bottleneck can be overcome by optimization of factors which influence the protein crystallizability. We demonstrated this approach on two scFv antibody fragments, and on prokaryotic metabolic regulator. We have performed protein pre-crystallization analysis employing the combination of size-exclusion chromatography, dynamic light scattering, and thermofluor assay. Based on the analysis data, we have developed crystallization protocols which yielded monocrystals suitable for X-ray diffraction analysis. This enabled us to determine crystal structure of the effector-binding domain of DeoR repressor.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FR - Pharmacology and apothecary chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Materials Structure in Chemistry, Biology, Physics and Technology

  • ISSN

    1211-5894

  • e-ISSN

  • Volume of the periodical

    20

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    3

  • Pages from-to

    121-123

  • UT code for WoS article

  • EID of the result in the Scopus database