In Vitro Transfection Mediated by Dendrigraft Poly(L-lysines): The Effect of Structure and Molecule Size
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00392354" target="_blank" >RIV/61388963:_____/13:00392354 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/13:10173586
Result on the web
<a href="http://dx.doi.org/10.1002/mabi.201200303" target="_blank" >http://dx.doi.org/10.1002/mabi.201200303</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mabi.201200303" target="_blank" >10.1002/mabi.201200303</a>
Alternative languages
Result language
angličtina
Original language name
In Vitro Transfection Mediated by Dendrigraft Poly(L-lysines): The Effect of Structure and Molecule Size
Original language description
Dendritic poly(L-lysines) (DGL) constitute promising nanomaterials applicable as a nonviral gene-delivery vector. In this study, we evaluate the transfection abilities of four DGL generations with special emphasis on the systematic description of the relationship of how generation (i.e., molecule size) affects the transfection efficacy. Using Hep2 cells, we demonstrated that the capability of unmodified DGL to deliver plasmid is of a magnitude lower than that of jetPEI. On the other hand, employing theHep2 cell line stably transduced with eGFP, we observed that DGL G5 delivers the siRNA oligonucleotide with the same efficiency as Lipofectamine 2000. In further experiments, it was shown that DGL affords excellent ability to bind DNA, protect it againstDNase I attack, and internalize it into cells.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecular Bioscience
ISSN
1616-5187
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
167-176
UT code for WoS article
000315298100006
EID of the result in the Scopus database
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