Mouse Tcf3 represses canonical Wnt signaling by either competing for beta-catenin binding or through occupation of DNA-binding sites

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F12%3A00387847" target="_blank" >RIV/68378050:_____/12:00387847 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s11010-012-1243-9" target="_blank" >http://dx.doi.org/10.1007/s11010-012-1243-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-012-1243-9" target="_blank" >10.1007/s11010-012-1243-9</a>

Result language

angličtina

Original language name

Mouse Tcf3 represses canonical Wnt signaling by either competing for beta-catenin binding or through occupation of DNA-binding sites

Original language description

Tcf3 acts as a transcription factor controlling gene expression in canonical Wnt signaling. In this study we show that mouse Tcf3 represses canonical Wnt signaling in mouse neural stem cells and in human HEK 293 cells. We demonstrate that mouse Tcf3 mediates repression of both moderate and high levels of canonical Wnt signaling, by either competing with other members of the Tcf/Lef family for binding to beta-catenin, or for binding to DNA. We observed that the repressor activity of mouse Tcf3 was only relieved effectively upon simultaneous disruption of both mechanisms. Immunofluorescence of transfected HEK 293 cells showed co-localization of beta-catenin and Tcf3 in the nucleus of cells transfected with full-length Tcf3, but not in cells transfected with N-terminal deleted versions. A direct physical interaction between beta-catenin and Tcf3 in the nucleus was confirmed by co-immunoprecipitation studies. The inhibitory beta-catenin/Tcf3 interface was independent of the ability of Tcf3

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

—

Volume of the periodical

365

Issue of the periodical within the volume

1-2

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

53-63

UT code for WoS article

000303472400007

EID of the result in the Scopus database

—