Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F07%3A00094469" target="_blank" >RIV/68378050:_____/07:00094469 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Abnormal lens morphogenesis and ectopic lens formation in the absence of beta-catenin function

Original language description

Beta-Catenin plays a key role in cadherin-mediated cell adhesion as well as in canonical Wnt signaling. To study the role of beta-catenin during eye development, we used conditional Cre/loxP system in mouse to inactivate beta-catenin in developing lens and retina. Inactivation of beta-catenin does not suppress lens fate, but instead results in abnormal morphogenesis of the lens. Using BAT-gal reporter mice, we show that beta-catenin-mediated Wnt signaling is notably absent from lens and neuroretina throughout eye development. The observed defect is therefore likely due to the cytoskeletal role of beta-catenin, and is accompanied by impaired epithelial cell adhesion. In contrast, inactivation of beta-catenin in the nasal ectoderm, an area with active Wnt signaling, results in formation of crystallin-positive ectopic lentoid bodies. These data suggest that, outside of the normal lens, beta-catenin functions as a coactivator of canonical Wnt signaling to suppress lens fate.

Czech name

Abnormální morfogeneze a ektopická tvorba čočky v nepřítomnosti funkce beta-kateninu

Czech description

Beta-catenin hraje klíčovou roli při adhezi buněk stejně jako v kanonické signální dráze wnt. Ke studiu role beta-cateninu během očního rozvoje jsme použili podmínečné CRE / loxp myši. Inaktivace beta-cateninu nikterak nepotlačí vznik čočky, ale má za následek abnormální morphogenezi. Naproti tomu, inaktivace beta-cateninu v nosním ectodermu, v oblasti s aktivními wnt signály, má za následek utváření crystallin-pozitivních ectopických čoček.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F04%2F1358" target="_blank" >GA204/04/1358: The role of wnt signalling in eye development and lens cell differentiation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genesis

ISSN

1526-954X

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

157-168

UT code for WoS article

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EID of the result in the Scopus database

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