Ectopic Activation of Wnt/beta-Catenin Signaling in Lens Fiber Cells Results in Cataract Formation and Aberrant Fiber Cell Differentiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10159326" target="_blank" >RIV/00216208:11310/13:10159326 - isvavai.cz</a>

Result on the web

<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078279" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0078279</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0078279" target="_blank" >10.1371/journal.pone.0078279</a>

Result language

angličtina

Original language name

Ectopic Activation of Wnt/beta-Catenin Signaling in Lens Fiber Cells Results in Cataract Formation and Aberrant Fiber Cell Differentiation

Original language description

The Wnt/beta-catenin signaling pathway controls many processes during development, including cell proliferation, cell differentiation and tissue homeostasis, and its aberrant regulation has been linked to various pathologies. In this study we investigated the effect of ectopic activation of Wnt/beta-catenin signaling during lens fiber cell differentiation. To activate Wnt/beta-catenin signaling in lens fiber cells, the transgenic mouse referred to as alpha A-CLEF was generated, in which the transactivation domain of beta-catenin was fused to the DNA-binding protein LEF1, and expression of the transgene was controlled by alpha A-crystallin promoter. Constitutive activation of Wnt/beta-catenin signaling in lens fiber cells of alpha A-CLEF mice resulted in abnormal and delayed fiber cell differentiation. Moreover, adult alpha A-CLEF mice developed cataract, microphthalmia and manifested downregulated levels of gamma-crystallins in lenses. We provide evidence of aberrant expression of cell

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

10

Country of publishing house

ES - SPAIN

Number of pages

13

Pages from-to

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UT code for WoS article

000326334500108

EID of the result in the Scopus database

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