Saccharomyces cerevisiae can secrete Sapp1p proteinase of Candida parapsilosis but cannot use it for efficient nitrogen acquisition

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00394743" target="_blank" >RIV/61388963:_____/13:00394743 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/13:00394743

Result on the web

<a href="http://dx.doi.org/10.1007/s12275-013-2422-4" target="_blank" >http://dx.doi.org/10.1007/s12275-013-2422-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12275-013-2422-4" target="_blank" >10.1007/s12275-013-2422-4</a>

Result language

angličtina

Original language name

Saccharomyces cerevisiae can secrete Sapp1p proteinase of Candida parapsilosis but cannot use it for efficient nitrogen acquisition

Original language description

Secreted aspartic proteinase Sapp1p of Candida parapsilosis represents one of the factors contributing to the pathogenicity of the fungus. The proteinase is synthesized as an inactive pre-pro-enzyme, but only processed Sapp1p is secreted into extracellular space. We constructed a plasmid containing the SAPP1 coding sequence under control of the ScGAL1 promoter and used it for proteinase expression in a Saccharomyces cerevisiae kex2 Delta mutant. Because Sapp1p maturation depends on cleavage by Kex2p proteinase, the kex2 Delta mutant secreted only the pro-form of Sapp1p. Characterization of this secreted proteinase form revealed that the Sapp1p signal peptide consists of 23 amino acids. Additionally, we prepared a plasmid with the SAPP1 coding sequenceunder control of its authentic CpSAPP1 promoter, which contains two GATAA motifs. While in C. parapsilosis SAPP1 expression is repressed by good low molecular weight nitrogen sources (e.g., ammonium ions), S. cerevisiae cells harboring th

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiology

ISSN

1225-8873

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

3

Country of publishing house

KR - KOREA, REPUBLIC OF

Number of pages

9

Pages from-to

336-344

UT code for WoS article

000321134100011

EID of the result in the Scopus database

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