One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00399957" target="_blank" >RIV/61388963:_____/13:00399957 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/13:43895626 RIV/60461373:22810/13:43895626 RIV/60461373:22330/13:43897411 RIV/60461373:22810/13:43897411

Result on the web

<a href="http://dx.doi.org/10.1016/j.pep.2013.09.003" target="_blank" >http://dx.doi.org/10.1016/j.pep.2013.09.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pep.2013.09.003" target="_blank" >10.1016/j.pep.2013.09.003</a>

Result language

angličtina

Original language name

One-step separation of myristoylated and nonmyristoylated retroviral matrix proteins

Original language description

N-terminal myristoylation of retroviral matrix proteins is essential for the targeting of the Gag polyproteins to the plasma membrane. To investigate the effect of the myristoylation on the structure and membrane binding ability of the matrix proteins, it is necessary to prepare their myristoylated forms. We present purification of myristoylated matrix proteins of the mouse mammary tumor virus and murine leukemia virus, two morphogenetically distinct retroviruses. The proteins were expressed in Escherichia coli coexpressing a yeast N-myristoyltransferase. This E. coli expression system yielded a mixture of myristoylated and nonmyristoylated matrix proteins. We established efficient one-step metal affinity purification that enabled to obtain pure myristoylated matrix proteins suitable for structural and functional studies.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA204%2F09%2F1388" target="_blank" >GA204/09/1388: Characterization of novel host cell protein interacting with protease of Mason-Pfizer monkey virus</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protein Expression and Purification

ISSN

1046-5928

e-ISSN

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Volume of the periodical

92

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

94-99

UT code for WoS article

000326257000014

EID of the result in the Scopus database

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