Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE
Result description
CZE has been applied to determination of thermodynamic acidity constants (pK(a)) of ionogenic groups and actual ionic mobilities of polyprotic peptides-synthetic human and salmon gonadotropin-releasing hormones and their derivatives and fragments. First,the mixed acidity constants, pK(a,i)(mix), of ionogenic groups, and actual ionic mobilities, m(i), of gonadotropin-releasing hormone peptides were determined by nonlinear regression analysis of pH dependence of their effective electrophoretic mobilities. The effective mobilities were measured by CZE in a series of BGEs within a broad pH range (1.80-12.10), at constant ionic strength (25 mM) and reference temperature (25 degrees C). Second, the pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of carboxyl groups was estimated to be in the range of 2.5-3.3 for C-terminal amino acids of the above peptides, and 5.2 for glutamic acid in the middle of peptide chain; pK(a) of imid
Keywords
acid dissociation constantgonadotropin-releasing hormonesionization constantpeptidespK(a)
The result's identifiers
Result code in IS VaVaI
Result on the web
DOI - Digital Object Identifier
Alternative languages
Result language
angličtina
Original language name
Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE
Original language description
CZE has been applied to determination of thermodynamic acidity constants (pK(a)) of ionogenic groups and actual ionic mobilities of polyprotic peptides-synthetic human and salmon gonadotropin-releasing hormones and their derivatives and fragments. First,the mixed acidity constants, pK(a,i)(mix), of ionogenic groups, and actual ionic mobilities, m(i), of gonadotropin-releasing hormone peptides were determined by nonlinear regression analysis of pH dependence of their effective electrophoretic mobilities. The effective mobilities were measured by CZE in a series of BGEs within a broad pH range (1.80-12.10), at constant ionic strength (25 mM) and reference temperature (25 degrees C). Second, the pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of carboxyl groups was estimated to be in the range of 2.5-3.3 for C-terminal amino acids of the above peptides, and 5.2 for glutamic acid in the middle of peptide chain; pK(a) of imid
Czech name
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Czech description
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Classification
Type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
34
Issue of the periodical within the volume
18
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
2655-2665
UT code for WoS article
000327590900008
EID of the result in the Scopus database
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Result type
Jx - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP
CB - Analytical chemistry, separation
Year of implementation
2013