Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00421961" target="_blank" >RIV/61388963:_____/13:00421961 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201300119" target="_blank" >http://dx.doi.org/10.1002/elps.201300119</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201300119" target="_blank" >10.1002/elps.201300119</a>

Result language

angličtina

Original language name

Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE

Original language description

CZE has been applied to determination of thermodynamic acidity constants (pK(a)) of ionogenic groups and actual ionic mobilities of polyprotic peptides-synthetic human and salmon gonadotropin-releasing hormones and their derivatives and fragments. First,the mixed acidity constants, pK(a,i)(mix), of ionogenic groups, and actual ionic mobilities, m(i), of gonadotropin-releasing hormone peptides were determined by nonlinear regression analysis of pH dependence of their effective electrophoretic mobilities. The effective mobilities were measured by CZE in a series of BGEs within a broad pH range (1.80-12.10), at constant ionic strength (25 mM) and reference temperature (25 degrees C). Second, the pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of carboxyl groups was estimated to be in the range of 2.5-3.3 for C-terminal amino acids of the above peptides, and 5.2 for glutamic acid in the middle of peptide chain; pK(a) of imid

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

34

Issue of the periodical within the volume

18

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

2655-2665

UT code for WoS article

000327590900008

EID of the result in the Scopus database

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