Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00470552" target="_blank" >RIV/61388963:_____/16:00470552 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201600342" target="_blank" >http://dx.doi.org/10.1002/elps.201600342</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201600342" target="_blank" >10.1002/elps.201600342</a>
Alternative languages
Result language
angličtina
Original language name
Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis
Original language description
Capillary electrophoresis (CE) was employed for the determination of thermodynamic acidity constants (pK(a)) and actual ionic mobilities of polycationic antimicrobial peptides (AMPs). The effective electrophoretic mobilities of AMPs were measured by CE in a series of the background electrolytes within a wide pH range (2.00-12.25), at constant ionic strength (25mM) and ambient temperature, using polybrene coated fused silica capillaries to suppress sorption of cationic AMPs to the capillary wall. Eventually, Haarhoff-Van der Linde peak fitting function was used for the determination of correct migration times of some AMPs peaks that were distorted by electromigration dispersion. The measured effective mobilities were corrected to 25 degrees C. Mixed acidity constants, pK(a,i)(mix), and actual ionic mobilities, m(i), of AMPs were determined by the nonlinear regression analysis of pH dependence of their effective mobilities. The pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of imidazolium group of histidine residues was found to be in the range 3.72-4.98, pK(a) of alpha-NH3+ group was in the range 6.14-6.93, and pK(a) of epsilon-NH3+ group of lysine spanned the interval 7.26-9.84, depending on the particular amino acid sequence of the AMPs. Actual ionic mobilities of AMPs with positive charges from one to six elementary units achieved values (9.8-36.5) x 10(-9) m(2)V(-1)s(-1).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA15-01948S" target="_blank" >GA15-01948S: Capillary electromigration techniques using affinity selectors & smart polymers for analysis and properties and interactions studies of biomolecules</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
37
Issue of the periodical within the volume
23/24
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
3186-3195
UT code for WoS article
000392421400019
EID of the result in the Scopus database
2-s2.0-84991671385