Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00427919" target="_blank" >RIV/61388963:_____/14:00427919 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/14:00427919
Result on the web
<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0093322" target="_blank" >http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0093322</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0093322" target="_blank" >10.1371/journal.pone.0093322</a>
Alternative languages
Result language
angličtina
Original language name
Delta 12-Fatty Acid Desaturase from Candida parapsilosis Is a Multifunctional Desaturase Producing a Range of Polyunsaturated and Hydroxylated Fatty Acids
Original language description
Numerous Delta 12-, Delta 15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Delta 12/Delta 15- and Delta 15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic yeast Candida parapsilosis. Overexpression of CpFad3 in Saccharomyces cerevisiae strains supplemented with linoleic acid (Delta 9,Delta 12-18:2) and hexadecadienoic acid (Delta 9,Delta 12-16:2) leads to accumulation of Delta 15-PUFAs, i.e., alpha-linolenic acid (Delta 9,Delta 12,Delta 15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (Delta 9,Delta 12,Delta 15-16:3). CpFad2 produces a range of Delta 12- and Delta 15-PUFAs. The major products of CpFad2 are linoleic and hexadecadienoic acid (Delta 9,Delta 12-16:2), accompanied by alpha-linolenic acid
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/TA01011461" target="_blank" >TA01011461: Immobilized yeasts in biotechnology: development of new applications for manufacturing</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"e93322/1"-"e93322/10"
UT code for WoS article
000333678100043
EID of the result in the Scopus database
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