Transport mechanisms of a novel antileukemic and antiviral compound 9-norbornyl-6-chloropurine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00443259" target="_blank" >RIV/61388963:_____/15:00443259 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3109/14756366.2013.879576" target="_blank" >http://dx.doi.org/10.3109/14756366.2013.879576</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/14756366.2013.879576" target="_blank" >10.3109/14756366.2013.879576</a>
Alternative languages
Result language
angličtina
Original language name
Transport mechanisms of a novel antileukemic and antiviral compound 9-norbornyl-6-chloropurine
Original language description
6-Chloropurines substituted at the position 9 with variously modified bicyclic skeletons represent promising antiviral and anticancer agents. This work aimed to investigate the transport mechanisms of 9-[(1R*,2R*,4S*)-bicyclo[2.2.1]hept-2-yl]-6-chloro-9H-purine (9-norbornyl-6-chloropurine, NCP) and their relationship to the metabolism and biological activity of the compound. Transport experiments were conducted in CCRF-CEM cells using radiolabeled compound ([H-3]NCP). The pattern of the intracellular uptake of [H-3]NCP in CCRF-CEM cells pointed to a combination of passive and facilitated diffusion as prevailing transport mechanisms. NCP intracellular metabolism was found to enhance its uptake by modifying NCP concentration gradient. The transport kinetics reached steady state under the conditions of MRP and MDR proteins blockade, indicating that NCP is a substrate for these efflux pumps. Their inhibition also increased the cytotoxicity of NCP. Our findings suggest that the novel nucleo
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP303%2F11%2F1297" target="_blank" >GAP303/11/1297: Mechanisms of antiviral and cytostatic effects of novel carbocyclic nucleoside analogs</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Enzyme Inhibition and Medicinal Chemistry
ISSN
1475-6366
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
57-62
UT code for WoS article
000347956500009
EID of the result in the Scopus database
2-s2.0-84921294824