Synthesis of Derivatized Chitooligomers using Transglycosidases Engineered from the Fungal GH20 beta-N-Acetylhexosaminidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00445811" target="_blank" >RIV/61388963:_____/15:00445811 - isvavai.cz</a>

Alternative codes found

RIV/67179843:_____/15:00445811 RIV/61388971:_____/15:00445811

Result on the web

<a href="http://dx.doi.org/10.1002/adsc.201500075" target="_blank" >http://dx.doi.org/10.1002/adsc.201500075</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adsc.201500075" target="_blank" >10.1002/adsc.201500075</a>

Result language

angličtina

Original language name

Synthesis of Derivatized Chitooligomers using Transglycosidases Engineered from the Fungal GH20 beta-N-Acetylhexosaminidase

Original language description

The synthesis of oligosaccharides using mutant glycosidases has been dynamically developing due to the need for novel carbohydrate-based materials. Chitooligomers (beta-1 -> 4-linked oligomers of N-acetylglucosamine) are bioactive compounds applicable inmany industrial and pharmacological areas; however, their accessibility is still rather low. In this work, GH20 beta-N-acetylhexosaminidase from the fungus Talaromyces flavus was engineered by site-directed mutagenesis to obtain three efficiently transglycosylating variants with ca. 200-times suppressed hydrolytic activity. Thus, we have prepared the first GH20 transglycosidases. In the reactions catalyzed by these mutant beta-N-acetylhexosaminidases we were able to easily prepare and isolate both natural and modified chitooligomers in sufficient amounts for their complete spectral characterization and possible further application. The presented method for the synthesis of chitooligomers with aglycones suitable for linking to other bio

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GP13-06818P" target="_blank" >GP13-06818P: New synthetic approaches using mutant and wild-type beta-N-acetylhexosaminidase from Talaromyces flavus</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Advanced Synthesis & Catalysis

ISSN

1615-4150

e-ISSN

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Volume of the periodical

357

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

1941-1950

UT code for WoS article

000355235700033

EID of the result in the Scopus database

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