Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00454168" target="_blank" >RIV/61388963:_____/15:00454168 - isvavai.cz</a>

Alternative codes found

RIV/68378050:_____/15:00454168

Result on the web

<a href="http://dx.doi.org/10.1107/S1399004715019392" target="_blank" >http://dx.doi.org/10.1107/S1399004715019392</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S1399004715019392" target="_blank" >10.1107/S1399004715019392</a>

Result language

angličtina

Original language name

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis

Original language description

The virulence of the Candida pathogens is enhanced by the production of secreted aspartic proteases, which therefore represent possible targets for drug design. Here, the crystal structure of the secreted aspartic protease Sapp2p from Candida parapsilosis was determined. Sapp2p was isolated from its natural source and crystallized in complex with pepstatin A, a classical aspartic protease inhibitor. The atomic resolution of 0.83 angstrom allowed the protonation states of the active-site residues to be inferred. A detailed comparison of the structure of Sapp2p with the structure of Sapp1p, the most abundant C. parapsilosis secreted aspartic protease, was performed. The analysis, which included advanced quantum-chemical interaction-energy calculations, uncovered molecular details that allowed the experimentally observed equipotent inhibition of both isoenzymes by pepstatin A to be rationalized.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GA14-23022S" target="_blank" >GA14-23022S: Structural studies of aspartic protease from Candida parapsilosis as a tool for design of antimycotic compounds.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Crystallographica Section D-Biological Crystalloghraphy

ISSN

1399-0047

e-ISSN

—

Volume of the periodical

71

Issue of the periodical within the volume

12

Country of publishing house

DK - DENMARK

Number of pages

11

Pages from-to

2494-2504

UT code for WoS article

000365773900012

EID of the result in the Scopus database

2-s2.0-84948798309