FAITH - Fast Assembly Inhibitor Test for HIV

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00454191" target="_blank" >RIV/61388963:_____/15:00454191 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/15:43899470

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0042682215003864" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0042682215003864</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.virol.2015.08.029" target="_blank" >10.1016/j.virol.2015.08.029</a>

Result language

angličtina

Original language name

FAITH - Fast Assembly Inhibitor Test for HIV

Original language description

Due to the high number of drug-resistant HIV-1 mutants generated by highly active antiretroviral therapy (HAART), there is continuing demand for new types of inhibitors. Both the assembly of the Gag polyprotein into immature and mature HIV-1 particles are attractive candidates for the blocking of the retroviral life cycle. Currently, no therapeutically-used assembly inhibitor is available. One possible explanation is the lack of a reliable and simple assembly inhibitor screening method. To identify compounds potentially inhibiting the formation of both types of HIV-1 particles, we developed a new fluorescent high-throughput screening assay. This assay is based on the quantification of the assembly efficiency in vitro in a 96-well plate format. The keycomponents of the assay are HIV-1 Gag-derived proteins and a dual-labelled oligonucleotide, which emits fluorescence only when the assembly of retroviral particles is inhibited. The method was validated using three (CAI, BM2, FF74) report

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virology

ISSN

0042-6822

e-ISSN

—

Volume of the periodical

486

Issue of the periodical within the volume

Dec

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

78-87

UT code for WoS article

000366229800012

EID of the result in the Scopus database

2-s2.0-84942783664