6-Aryl-4-amino-pyrimido[4,5-b]indole 2'-deoxyribonucleoside triphosphates (benzo-fused 7-deaza-dATP analogues): Synthesis, fluorescent properties, enzymatic incorporation into DNA and DNA-protein binding study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00464456" target="_blank" >RIV/61388963:_____/16:00464456 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10328266
Result on the web
<a href="http://dx.doi.org/10.1016/j.bmc.2016.07.054" target="_blank" >http://dx.doi.org/10.1016/j.bmc.2016.07.054</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bmc.2016.07.054" target="_blank" >10.1016/j.bmc.2016.07.054</a>
Alternative languages
Result language
angličtina
Original language name
6-Aryl-4-amino-pyrimido[4,5-b]indole 2'-deoxyribonucleoside triphosphates (benzo-fused 7-deaza-dATP analogues): Synthesis, fluorescent properties, enzymatic incorporation into DNA and DNA-protein binding study
Original language description
Four 6-substituted 4-amino-pyrimido[4,5-b]indole 2'-deoxyribonucleoside triphosphates (dA(BX)TPs) were prepared by glycosylation of 4,6-dichloropyrimidoindole followed by ammonolysis, cross-coupling and triphosphorylation. They were found to be moderate to good substrates for DNA polymerases in primer extension. They also exerted fluorescence with emission maxima 335-378 nm. When incorporated to oligonucleotide probes, they did not show significant mismatch discrimination but the 6-benzofuryl 4-amino-pyrimido[4,5-b]indole nucleotide displayed a useful sensitivity to protein binding in experiment with SSB protein.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA16-00178S" target="_blank" >GA16-00178S: Novel nucleosides and nucleotides derived from hetero-fused 7-deazapurines</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioorganic & Medicinal Chemistry
ISSN
0968-0896
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
19
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
4528-4535
UT code for WoS article
000383732200002
EID of the result in the Scopus database
2-s2.0-84986004176