Immunoassay for determination of trilobolide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00474379" target="_blank" >RIV/61388963:_____/17:00474379 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/17:00474379 RIV/00216208:11140/17:10330171 RIV/60461373:22330/17:43902751 RIV/60461373:22810/17:43902751 RIV/00023761:_____/17:N0000012
Result on the web
<a href="http://dx.doi.org/10.1016/j.steroids.2016.08.019" target="_blank" >http://dx.doi.org/10.1016/j.steroids.2016.08.019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.steroids.2016.08.019" target="_blank" >10.1016/j.steroids.2016.08.019</a>
Alternative languages
Result language
angličtina
Original language name
Immunoassay for determination of trilobolide
Original language description
Trilobolide (Tb) is a pharmacologically interesting sesquiterpene lactone isolated from Laser trilobum (L.) Borkh. Structural relation to a sarco/endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin bring promising prospects for Tb to be used in the development of new anti-cancer drugs. As long as there are still unanswered questions regarding its investigation, a need for novel analytical tools emerge. Since immunoassays serve as one of powerful tools within the investigation of natural products, the development of indirect competitive enzyme-linked immunosorbent assay (ELISA) utilizing coating based on avidin-biotin technology is described. In our set-up of ELISA, newly synthesized biotinylated Tb served as immobilized competitor. Tb-carboxymethyloxime-bovine serum albumin (BSA) and Tb-succinoyl-BSA conjugates were used separately for immunization of rabbits. Two sets of polyclonal antibodies (RAbs) were obtained. Antibodies against Tb-succinoyl-BSA conjugate (RAb No. 206) were chosen as the best. Under optimized conditions, limit of detection and 50% intercept of our ELISA were 849 pg/mL and 8.89 ng/mL, respectively. The cross-reactivity (CR) was tested on 10 structurally related compounds and CR did not exceed 6.1%. The reproducibility of the system is expressed as intra- and inter-assay coefficients of variation (9.7% and 11.4%, respectively). Based on conducted experiments, we proposed the use of ELISA for quantification of Tb in complex biological matrices such as plant extracts. A method was applied to analyze three extracts obtained from different parts of L trilobum. Data obtained were compared to those acquired by UHPLC-MS/MS. The concordance between the methods (103-87%) showed the ability of ELISA to quantify Tb.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/GA14-04329S" target="_blank" >GA14-04329S: Relationship between biological activities of sesquiterpene lactones to their subcellular localization</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Steroids
ISSN
0039-128X
e-ISSN
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Volume of the periodical
117
Issue of the periodical within the volume
Jan
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
105-111
UT code for WoS article
000392566800015
EID of the result in the Scopus database
2-s2.0-84995488385