Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00474778" target="_blank" >RIV/61388963:_____/17:00474778 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/srep41839" target="_blank" >https://www.nature.com/articles/srep41839</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/srep41839" target="_blank" >10.1038/srep41839</a>
Alternative languages
Result language
angličtina
Original language name
Molecular mutagenesis of ppGpp: turning a RelA activator into an inhibitor
Original language description
The alarmone nucleotide (p) ppGpp is a key regulator of bacterial metabolism, growth, stress tolerance and virulence, making (p) ppGpp-mediated signaling a promising target for development of antibacterials. Although ppGpp itself is an activator of the ribosome-associated ppGpp synthetase RelA, several ppGpp mimics have been developed as RelA inhibitors. However promising, the currently available ppGpp mimics are relatively inefficient, with IC50 in the sub-mM range. In an attempt to identify a potent and specific inhibitor of RelA capable of abrogating (p) ppGpp production in live bacterial cells, we have tested a targeted nucleotide library using a biochemical test system comprised of purified Escherichia coli components. While none of the compounds fulfilled this aim, the screen has yielded several potentially useful molecular tools for biochemical and structural work.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/GA15-11711S" target="_blank" >GA15-11711S: Development of a molecular toolkit for control and investigation of the bacterial stringent response</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
Feb 3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
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UT code for WoS article
000393552300001
EID of the result in the Scopus database
2-s2.0-85011591064