Conformational study of melectin and antapin antimicrobial peptides in model membrane environments
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00475296" target="_blank" >RIV/61388963:_____/17:00475296 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22320/17:43913252 RIV/60461373:22340/17:43913252
Result on the web
<a href="http://dx.doi.org/10.1016/j.saa.2016.07.015" target="_blank" >http://dx.doi.org/10.1016/j.saa.2016.07.015</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.saa.2016.07.015" target="_blank" >10.1016/j.saa.2016.07.015</a>
Alternative languages
Result language
angličtina
Original language name
Conformational study of melectin and antapin antimicrobial peptides in model membrane environments
Original language description
Antimicrobial peptides have long been considered as promising compounds against drug-resistant pathogens. In this work, we studied the secondary structure of antimicrobial peptides melectin and antapin using electronic (ECD) and vibrational circular dichroism (VCD) spectroscopies that are sensitive to peptide secondary structures. The results from quantitative ECD spectral evaluation by Dichroweb and CDNN program and from the qualitative evaluation of the VCD spectra were compared. The antimicrobial activity of the selected peptides depends on their ability to adopt an amphipathic alpha-helical conformation on the surface of the bacterial membrane. Hence, solutions of different zwitterionic and negatively charged liposomes and micelles were used to mimic the eukaryotic and bacterial biological membranes. The results show a significant content of alpha-helical conformation in the solutions of negatively charged liposomes mimicking the bacterial membrane, thus correlating with the antimicrobial activity of the studied peptides. On the other hand in the solutions of zwitterionic liposomes used as models of the eukaryotic membranes, the fraction of alpha-helical conformation was lower, which corresponds with their moderate hemolytic activity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
ISSN
1386-1425
e-ISSN
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Volume of the periodical
170
Issue of the periodical within the volume
Jan 5
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
247-255
UT code for WoS article
000398746600033
EID of the result in the Scopus database
2-s2.0-84978405312