Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F17%3A00478474" target="_blank" >RIV/61388963:_____/17:00478474 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10363658

Result on the web

<a href="https://www.nature.com/articles/s41598-017-08303-4" target="_blank" >https://www.nature.com/articles/s41598-017-08303-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-017-08303-4" target="_blank" >10.1038/s41598-017-08303-4</a>

Result language

angličtina

Original language name

Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners

Original language description

CAS is a docking protein downstream of the proto-oncogene Src with a role in invasion and metastasis of cancer cells. The CAS SH3 domain is indispensable for CAS-mediated signaling, but structural aspects of CAS SH3 ligand binding and regulation are not well understood. Here, we identified the consensus CAS SH3 binding motif and structurally characterized the CAS SH3 domain in complex with ligand. We revealed the requirement for an uncommon centrally localized lysine residue at position +2 of CAS SH3 ligands and two rather dissimilar optional anchoring residues, leucine and arginine, at position +5. We further expanded the knowledge of CAS SH3 ligand binding regulation by manipulating tyrosine 12 phosphorylation and confirmed the negative role of this phosphorylation on CAS SH3 ligand binding. Finally, by exploiting the newly identified binding requirements of the CAS SH3 domain, we predicted and experimentally verified two novel CAS SH3 binding partners, DOK7 and GLIS2.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

Aug 14

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

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UT code for WoS article

000407559800024

EID of the result in the Scopus database

2-s2.0-85027462937