Sequence Requirements of Intrinsically Fluorescent G-Quadruplexes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00492073" target="_blank" >RIV/61388963:_____/18:00492073 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1021/acs.biochem.8b00252" target="_blank" >http://dx.doi.org/10.1021/acs.biochem.8b00252</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.biochem.8b00252" target="_blank" >10.1021/acs.biochem.8b00252</a>

Result language

angličtina

Original language name

Sequence Requirements of Intrinsically Fluorescent G-Quadruplexes

Original language description

G-Quadruplexes are four-stranded nucleic acid structures typically stabilized by GGGG tetrads. These structures are intrinsically fluorescent, which expands the known scope of nucleic acid function and raises the possibility that they could eventually be used as signaling components in label-free sensors constructed from DNA or RNA. In this study, we systematically investigated the effects of mutations in tetrads, loops, and overhanging nucleotides on the fluorescence intensity and maximum emission wavelength of >500 sequence variants of a reference DNA G-quadruplex. Some of these mutations modestly increased the fluorescence intensity of this G-quadruplex, while others shifted its maximum emission wavelength. Mutations that increased the fluorescence intensity were distinct from those that increased the maximum emission wavelength, suggesting a trade-off between these two biochemical properties. The fluorescence intensity and maximum emission wavelength were also correlated with multimeric state: the most fluorescent G-quadruplexes were monomers, while those with the highest maximum emission wavelengths typically formed dimeric structures. Oligonucleotides containing multiple G-quadruplexes were in some cases more fluorescent than those containing a single G-quadruplex, although this depended on the length and sequence of the spacer linking the G-quadruplexes. These experiments provide new insights into the properties of fluorescent G-quadruplexes and should aid in the development of improved label-free nucleic acid sensors.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemistry

ISSN

0006-2960

e-ISSN

—

Volume of the periodical

57

Issue of the periodical within the volume

28

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

4052-4062

UT code for WoS article

000439397900003

EID of the result in the Scopus database

2-s2.0-85048718208