Protected 2 '-deoxyribonucleoside triphosphate building blocks for the photocaging of epigenetic 5-(hydroxymethyl)cytosine in DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00492199" target="_blank" >RIV/61388963:_____/18:00492199 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/18:10378040
Result on the web
<a href="http://pubs.rsc.org/en/content/articlehtml/2018/ob/c8ob01106k" target="_blank" >http://pubs.rsc.org/en/content/articlehtml/2018/ob/c8ob01106k</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c8ob01106k" target="_blank" >10.1039/c8ob01106k</a>
Alternative languages
Result language
angličtina
Original language name
Protected 2 '-deoxyribonucleoside triphosphate building blocks for the photocaging of epigenetic 5-(hydroxymethyl)cytosine in DNA
Original language description
2'-Deoxyribonucleoside triphosphates (dNTPs) containing 5-(hydroxymethyl)cytosine (5hmC) protected with photocleavable groups (2-nitrobenzyl or 6-nitropiperonyl) were prepared and studied as substrates for the enzymatic synthesis of oligonucleotides and DNA containing a photocaged epigenetic 5hmC base. DNA probes containing photocaged or free 5hmC in the recognition sequence of restriction endonucleases were prepared and used for the study of the photorelease of caged DNA by UV or visible light at different wavelengths. The nitrobenzyl-protected dNTP was a slightly better substrate for DNA polymerases in primer extension or PCR, whereas the nitropiperonyl-protected nucleotide underwent slightly faster photorelease at 400 nm. However, both photocaged building blocks can be used in polymerase synthesis and the photorelease of 5hmC in DNA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
<a href="/en/project/GA17-03419S" target="_blank" >GA17-03419S: Artificial epigenetics: Development of a eukaryotic bioorthogonal transcription system for gene expression manipulation in the cell.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Organic & Biomolecular Chemistry
ISSN
1477-0520
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
30
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
5427-5432
UT code for WoS article
000440580100008
EID of the result in the Scopus database
2-s2.0-85051075625