2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00495370" target="_blank" >RIV/61388963:_____/18:00495370 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10383308

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201803973" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/chem.201803973</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chem.201803973" target="_blank" >10.1002/chem.201803973</a>

Result language

angličtina

Original language name

2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA

Original language description

A series of 2-alkylamino-2-deoxyadenosine triphosphates (dATP) was prepared and found to be substrates for the Therminator DNA polymerase, which incorporated only one modified nucleotide into the primer. Using a template encoding for two consecutive adenines, conditions were found for incorporation of either one or two modified nucleotides. In all cases, addition of a mixture of natural dNTPs led to primer extension resulting in site-specific single modification of DNA in the minor groove. The allylamino-substituted DNA was used for the thiol-ene addition, whereas the propargylamino-DNA for the CuAAC click reaction was used to label the DNA with a fluorescent dye in the minor groove. The approach was used to construct FRET probes for detection of oligonucleotides.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10401 - Organic chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemistry - A European Journal

ISSN

0947-6539

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

56

Country of publishing house

DE - GERMANY

Number of pages

4

Pages from-to

14938-14941

UT code for WoS article

000446826100013

EID of the result in the Scopus database

2-s2.0-85053475718