Nonhydroxylated 1-O-acylceramides in vernix caseosa
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F18%3A00498494" target="_blank" >RIV/61388963:_____/18:00498494 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21110/18:00325856 RIV/00216208:11110/18:10383320 RIV/00216208:11310/18:10383320 RIV/00064165:_____/18:10383320
Result on the web
<a href="http://dx.doi.org/10.1194/jlr.M088864" target="_blank" >http://dx.doi.org/10.1194/jlr.M088864</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1194/jlr.M088864" target="_blank" >10.1194/jlr.M088864</a>
Alternative languages
Result language
angličtina
Original language name
Nonhydroxylated 1-O-acylceramides in vernix caseosa
Original language description
Vernix caseosa, the waxy substance that coats the skin of newborn babies, has an extremely complex lipid composition. We have explored these lipids and identified nonhydroxylated 1-O-acylceramides (1-O-ENSs) as a new class of lipids in vernix caseosa. These ceramides mostly contain saturated C11-C38 ester-linked (1-O) acyls, saturated C12-C39 amide-linked acyls, and C16-C24 sphingoid bases. Because their fatty acyl chains are frequently branched, numerous molecular species were separable and detectable by HPLC/MS: we found more than 2,300 molecular species, 972 of which were structurally characterized. The most abundant 1-O-ENSs contained straight-chain and branched fatty acyls with 20, 22, 24, or 26 carbons in the 1-O position, 24 or 26 carbons in the N position, and sphingosine. The 1-O-ENSs were isolated using multistep TLC and HPLC and they accounted for 1% of the total lipid extract. The molecular species of 1-O-ENSs were separated on a C18 HPLC column using an acetonitrile/propan-2-ol gradient and detected by APCI-MS, and the structures were elucidated by high-resolution and tandem MS. Medium-polarity 1-O-ENSs likely contribute to the cohesiveness and to the waterproofing and moisturizing properties of vernix caseosa.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Lipid Research
ISSN
0022-2275
e-ISSN
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Volume of the periodical
59
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
2164-2173
UT code for WoS article
000448737400012
EID of the result in the Scopus database
2-s2.0-85055906337