Europium (III) as a Circularly Polarized Luminescence Probe of DNA Structure
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00501566" target="_blank" >RIV/61388963:_____/19:00501566 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-018-37680-7" target="_blank" >https://www.nature.com/articles/s41598-018-37680-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-37680-7" target="_blank" >10.1038/s41598-018-37680-7</a>
Alternative languages
Result language
angličtina
Original language name
Europium (III) as a Circularly Polarized Luminescence Probe of DNA Structure
Original language description
We report as a proof-of-concept the first application of circularly polarized luminescence (CPL) measured with a Raman optical activity (ROA) spectrometer to differentiate several DNA structures without need of sensitizing complexes. The ROA/CPL approach provides sufficiently high CPL intensity to use hydrated Eu3+ ions, thus avoiding DNA structural changes associated with binding of sensitizers and overcoming the sensitizer quenching issue. We showed that deoxyguanosine monophosphate (dGMP), single- and double-stranded DNA provide different CPL spectra, which could be used for their discrimination. Our results demonstrate that ROA/CPL method is a promising approach to measure CPL spectra of complex biomolecules when the use of sensitizers is not possible. The method can be extended to other biomolecules, such as proteins, lipids, sugars, etc.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
Jan 31
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
1068
UT code for WoS article
000457287000056
EID of the result in the Scopus database
2-s2.0-85060911640