Induced Lanthanide Circularly Polarized Luminescence as a Probe of Protein Fibrils
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00503693" target="_blank" >RIV/61388963:_____/19:00503693 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/19:73594114
Result on the web
<a href="https://pubs.acs.org/doi/full/10.1021/acsomega.8b03175" target="_blank" >https://pubs.acs.org/doi/full/10.1021/acsomega.8b03175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsomega.8b03175" target="_blank" >10.1021/acsomega.8b03175</a>
Alternative languages
Result language
angličtina
Original language name
Induced Lanthanide Circularly Polarized Luminescence as a Probe of Protein Fibrils
Original language description
Protein fibrils are involved in a number of biological processes. Because their structure is very complex and not completely understood, different spectroscopic methods are used to monitor different aspects of fibril structure. We have explored circularly polarized luminescence (CPL) induced in lanthanide compounds to indicate fibril growth and discriminate among fibril types. For hen egg-white lysozyme and polyglutamic acid-specific CPL, spectral patterns were obtained and could be correlated with vibrational circular dichroism (VCD) spectra and thioflavin T fluorescence. The CPL spectra were measured on a Raman optical activity spectrometer, and its various polarization modes are discussed. The experiments indicate that the induced CPL is sensitive to more local aspects of the fibril structure than VCD. For CPL, smaller amounts of the sample are required for the analysis, and thus this method appears to be a good candidate for future spectroscopic characterization of these peptide and protein aggregates.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Omega
ISSN
2470-1343
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1265-1271
UT code for WoS article
000460214700138
EID of the result in the Scopus database
2-s2.0-85060169371