Inhibitor-Polymer Conjugates as a Versatile Tool for Detection and Visualization of Cancer-Associated Carbonic Anhydrase Isoforms
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00505496" target="_blank" >RIV/61388963:_____/19:00505496 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/19:00505496 RIV/68378050:_____/19:00523062 RIV/00216208:11310/19:10405474
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acsomega.9b00596" target="_blank" >https://pubs.acs.org/doi/10.1021/acsomega.9b00596</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsomega.9b00596" target="_blank" >10.1021/acsomega.9b00596</a>
Alternative languages
Result language
angličtina
Original language name
Inhibitor-Polymer Conjugates as a Versatile Tool for Detection and Visualization of Cancer-Associated Carbonic Anhydrase Isoforms
Original language description
Carbonic anhydrases (CAs) are zinc metalloenzymes that catalyze hydration of carbon dioxide to bicarbonate. They play a crucial role in a number of important physiological processes. CAIX is a tumor-associated transmembrane isoenzyme that is overexpressed on the cell surface in hypoxic solid tumors and represents a validated target for cancer therapy and diagnosis. Here, we describe development of an N-(2-hydroxypropyl)methacrylamide-based copolymer conjugate (iBody) containing a CAIX inhibitor for selective targeting of this enzyme on tumor cells. The iBody binds CAIX with sufficient affinity for use in biochemical applications. We show that the iBody can be used to isolate CAIX from cell lysates and to visualize it by flow cytometry and confocal microscopy. Our results demonstrate that the iBody is a valuable tool for targeting CAIX in vitro and with further development may serve as a platform for CAIX targeting in vivo.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Omega
ISSN
2470-1343
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
6746-6756
UT code for WoS article
000466552500073
EID of the result in the Scopus database
2-s2.0-85064397173