Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00505762" target="_blank" >RIV/61388963:_____/19:00505762 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/19:10394756 RIV/00064203:_____/19:10394756

Result on the web

<a href="https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001000" target="_blank" >https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.001000</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/jmm.0.001000" target="_blank" >10.1099/jmm.0.001000</a>

Result language

angličtina

Original language name

Antimicrobial peptides prevent bacterial biofilm formation on the surface of polymethylmethacrylate bone cement

Original language description

Purpose: Antibiotic-loaded polymethylmethacrylate-based bone cement has been implemented in orthopaedics to cope with implant-related infections associated with the formation of bacterial biofilms. In the context of emerging bacterial resistance to current antibiotics, we examined the efficacy of short antimicrobial peptide-loaded bone cement in inhibiting bacterial adhesion and consequent biofilm formation on its surface. Methodology: The ability of alpha-helical antimicrobial peptides composed of 12 amino acid residues to prevent bacterial biofilm [methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli] formation on the surface of model implants made from polymethylmethacrylate-based bone cement was evaluated by colony-forming unit (c.f) counting of bacteria released by sonication from the biofilms formed on their surfaces. The biofilms on model implant surfaces were also visualized by light microscopy after staining with tetrazolium dye (MTT) and by scanning electron microscopy. Results: When incorporated in the implants, these peptides caused a mean reduction in the number of bacterial cells attached to implants' surfaces (by five orders of magnitude), and 88 % of these implants showed no bacterial adhesion after being exposed to growth media containing various bacteria. Conclusion: The results showed that the antibiofilm activity of these peptides was comparable to that of the antibiotics, but the peptides exhibited broader specificity than the antibiotics. Given the rapid development of antibiotic resistance, antimicrobial peptides show promise as a substitute for antibiotics for loading into bone cements.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NV16-27726A" target="_blank" >NV16-27726A: Novel antimicrobial peptides for topical treatment of osteomyelitis and prevention of implant-related infections in orthopedics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Medical Microbiology

ISSN

0022-2615

e-ISSN

—

Volume of the periodical

68

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

961-972

UT code for WoS article

000470083100019

EID of the result in the Scopus database

2-s2.0-85067089662