Antimicrobial peptide in polymethylmethacrylate bone cement as a prophylaxis of infectious complications in orthopedics–an experiment in a murine model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00557909" target="_blank" >RIV/61388963:_____/22:00557909 - isvavai.cz</a>

Alternative codes found

RIV/00064203:_____/22:10443993 RIV/00216208:11110/22:10443993 RIV/00216208:11130/22:10443993

Result on the web

<a href="https://doi.org/10.1007/s12223-022-00979-0" target="_blank" >https://doi.org/10.1007/s12223-022-00979-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-022-00979-0" target="_blank" >10.1007/s12223-022-00979-0</a>

Result language

angličtina

Original language name

Antimicrobial peptide in polymethylmethacrylate bone cement as a prophylaxis of infectious complications in orthopedics–an experiment in a murine model

Original language description

Polymethylmethacrylate (PMMA) bone cement mixed with antibiotics is used in orthopedic surgery to cope with implant-related infections which are typically associated with the formation of bacterial biofilms. Taking into account the growing bacterial resistance to current antibiotics, we examined here the efficacy of a selected antimicrobial peptide (AMP) mixed into the bone cement to inhibit bacterial adhesion and the consequent biofilm formation on its surface. In particular, we followed the formation of bacterial biofilms of methicillin-resistant Staphylococcus aureus (MRSA) on implants made from PMMA bone cement loaded with AMP composed of 12 amino acid residues. This was evaluated by CFU counting of bacteria released by sonication from the biofilms formed on their surfaces after these implants were retrieved from the infected murine femoral canals. The AMP loaded in these model implants prevented adhesion of MRSA and the subsequent formation of MRSA biofilm on the surfaces of more than 80% of these implants, whereas biofilms did form on control implants made from the plain cement. The results of our experiments performed in the murine femoral canal indicate the potential for this murine osteomyelitis model to mimic actual operations in orthopedics.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/NV16-27726A" target="_blank" >NV16-27726A: Novel antimicrobial peptides for topical treatment of osteomyelitis and prevention of implant-related infections in orthopedics</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

1874-9356

Volume of the periodical

67

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

785-791

UT code for WoS article

000802309600001

EID of the result in the Scopus database

2-s2.0-85130760012