Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00507822" target="_blank" >RIV/61388963:_____/19:00507822 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/19:43918444
Result on the web
<a href="https://academic.oup.com/femsyr/article-abstract/19/3/foz013/5315757?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/femsyr/article-abstract/19/3/foz013/5315757?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsyr/foz013" target="_blank" >10.1093/femsyr/foz013</a>
Alternative languages
Result language
angličtina
Original language name
Antifungal activity of analogues of antimicrobial peptides isolated from bee venoms against vulvovaginal Candida spp.
Original language description
Candida albicans is the main causative agent of vulvovaginal candidiasis (VVC), a common mycosis in women, relapses of which are difficult to manage due to biofilm formation. This study aimed at developing novel non-toxic compounds active against Candida spp. biofilms. We synthesised analogues of natural antifungal peptides LL-III (LL-III/43) and HAL-2 (peptide VIII) originally isolated from bee venoms and elucidated their structures by nuclear magnetic resonance spectroscopy. The haemolytic, cytotoxic, antifungal and anti-biofilm activities of LL-III/43 and peptide VIII were then tested. LL-III/43 and VIII showed moderate cytotoxicity to HUVEC-2 cells and had comparable inhibitory activity against C. albicans and non-albicans spp. The lowest minimum inhibitory concentration (MIC90) of LL-III/43 was observed towards Candida tropicalis (0.8 mu M). That was 8-fold lower than that of antimycotic amphotericin B. Both peptides can be used to inhibit Candida spp. biofilm formation. Biofilm inhibitory concentrations (BIC50) ranged from 0.9 to 58.6 mu M and biofilm eradication concentrations (BEC50) for almost all tested Candida spp. strains ranged from 12.8 to 200 mu M. Also proven were the peptides' abilities to reduce the area colonised by biofilms, inhibit hyphae formation and permeabilise cell membranes in biofilms. LL-III/43 and VIII are promising candidates for further development as therapeutics against VVC.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS Yeast Research
ISSN
1567-1356
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
foz013
UT code for WoS article
000474809700004
EID of the result in the Scopus database
2-s2.0-85064107631