Squaramate-Modified Nucleotides and DNA for Specific Cross-Linking with Lysine-Containing Peptides and Proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00508774" target="_blank" >RIV/61388963:_____/19:00508774 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/19:10396908
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201906737" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201906737</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201906737" target="_blank" >10.1002/anie.201906737</a>

Result language
angličtina
Original language name
Squaramate-Modified Nucleotides and DNA for Specific Cross-Linking with Lysine-Containing Peptides and Proteins
Original language description
Squaramate-linked 2'-deoxycytidine 5'-O-triphosphate was synthesized and found to be good substrate for KOD XL DNA polymerase in primer extension or PCR synthesis of modified DNA. The resulting squaramate-linked DNA reacts with primary amines to form a stable diamide linkage. This reaction was used for bioconjugations of DNA with Cy5 and Lys-containing peptides. Squaramate-linked DNA formed covalent cross-links with histone proteins. This reactive nucleotide has potential for other bioconjugations of nucleic acids with amines, peptides or proteins without need of any external reagent.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10401 - Organic chemistry

Project
<a href="/en/project/GA18-03305S" target="_blank" >GA18-03305S: Bioconjugation reactions for cross-linking of proteins to DNA</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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