Azidopropylvinylsulfonamide as a New Bifunctional Click Reagent for Bioorthogonal Conjugations: Application for DNA-Protein Cross-Linking
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314365" target="_blank" >RIV/00216208:11310/15:10314365 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/15:00453444 RIV/61388963:_____/15:00453444 RIV/00216224:14740/15:00081380
Result on the web
<a href="http://dx.doi.org/10.1002/chem.201502209" target="_blank" >http://dx.doi.org/10.1002/chem.201502209</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/chem.201502209" target="_blank" >10.1002/chem.201502209</a>
Alternative languages
Result language
angličtina
Original language name
Azidopropylvinylsulfonamide as a New Bifunctional Click Reagent for Bioorthogonal Conjugations: Application for DNA-Protein Cross-Linking
Original language description
N-(3-Azidopropyl)vinylsulfonamide was developed as a new bifunctional bioconjugation reagent suitable for the cross-linking of biomolecules through copper(I)-catalyzed azide-alkyne cycloaddition and thiol Michael addition reactions under biorthogonal conditions. The reagent is easily clicked to an acetylene-containing DNA or protein and then reacts with cysteine-containing peptides or proteins to form covalent cross-links. Several examples of bioconjugations of ethynyl- or octadiynyl-modified DNA with peptides, p53 protein, or alkyne-modified human carbonic anhydrase with peptides are given.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemistry - A European Journal
ISSN
0947-6539
e-ISSN
—
Volume of the periodical
21
Issue of the periodical within the volume
45
Country of publishing house
DE - GERMANY
Number of pages
12
Pages from-to
16091-16102
UT code for WoS article
000363890700030
EID of the result in the Scopus database
2-s2.0-84945476915