Chloroacetamide-Linked Nucleotides and DNA for Cross-Linking with Peptides and Proteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F16%3A00464736" target="_blank" >RIV/68081707:_____/16:00464736 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/16:00464736 RIV/00216224:14740/16:00088768 RIV/00216208:11310/16:10328141
Result on the web
<a href="http://pubs.acs.org/doi/full/10.1021/acs.bioconjchem.6b00342" target="_blank" >http://pubs.acs.org/doi/full/10.1021/acs.bioconjchem.6b00342</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.bioconjchem.6b00342" target="_blank" >10.1021/acs.bioconjchem.6b00342</a>
Alternative languages
Result language
angličtina
Original language name
Chloroacetamide-Linked Nucleotides and DNA for Cross-Linking with Peptides and Proteins
Original language description
Nucleotides, 2'-deoxyribonucleoside triphosphates (dNTPs), and DNA probes bearing reactive chloroacetamido group linked to nucleobase (cytosine or 7-deazadaenine) through a propargyl tether were prepared and tested in cross-linking with cysteine- or histidine-containing peptides and proteins. The chloroacetamide-modifed dNTPs proved to be good substrates for DNA polymerases in the enzymatic synthesis of modified DNA probes. Modified nucleotides and DNA reacted efficiently with cysteine and cysteine-containing peptides, whereas the reaction with histidine was sluggish and low yielding. The modified DNA efficiently cross-linked with p53 protein through alkylation of cysteine and showed potential for cross-linking with histidine (in C277H mutant of p53).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioconjugate Chemistry
ISSN
1043-1802
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
2089-2094
UT code for WoS article
000384036900016
EID of the result in the Scopus database
2-s2.0-84988660482