A simple, high-throughput stabilization assay to test HIV-1 uncoating inhibitors

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00517403" target="_blank" >RIV/61388963:_____/19:00517403 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/19:43919093 RIV/60461373:22810/19:43919093

Result on the web

<a href="https://www.nature.com/articles/s41598-019-53483-w" target="_blank" >https://www.nature.com/articles/s41598-019-53483-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-019-53483-w" target="_blank" >10.1038/s41598-019-53483-w</a>

Result language

angličtina

Original language name

A simple, high-throughput stabilization assay to test HIV-1 uncoating inhibitors

Original language description

Shortly after entering the cell, HIV-1 copies its genomic RNA into double-stranded DNA in a process known as reverse transcription. This process starts inside a core consisting of an enclosed lattice of capsid proteins that protect the viral RNA from cytosolic sensors and degradation pathways. To accomplish reverse transcription and integrate cDNA into the host cell genome, the capsid shell needs to be disassembled, or uncoated. Premature or delayed uncoating attenuates reverse transcription and blocks HIV-1 infectivity. Small molecules that bind to the capsid lattice of the HIV-1 core and either destabilize or stabilize its structure could thus function as effective HIV-1 inhibitors. To screen for such compounds, we modified our recently developed FAITH assay to allow direct assessment of the stability of in vitro preassembled HIV-1 capsid-nucleocapsid (CANC) tubular particles. This new assay is a high-throughput fluorescence method based on measuring the amount of nucleic acid released from CANC complexes under disassembly conditions. The amount of disassembled CANC particles and released nucleic acid is proportional to the fluorescence signal, from which the relative percentage of CANC stability can be calculated. We consider our assay a potentially powerful tool for in vitro screening for compounds that alter HIV disassembly.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA17-25602S" target="_blank" >GA17-25602S: Study of the role of capsid protein in early phase of HIV-1 replication cycle using the newly identified compounds stabilizing capsid complexes</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

Nov 19

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

17076

UT code for WoS article

000497701800033

EID of the result in the Scopus database

2-s2.0-85075205104