Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00521503" target="_blank" >RIV/61388963:_____/20:00521503 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10420135
Result on the web
<a href="https://www.mdpi.com/1422-0067/21/3/850" target="_blank" >https://www.mdpi.com/1422-0067/21/3/850</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21030850" target="_blank" >10.3390/ijms21030850</a>
Alternative languages
Result language
angličtina
Original language name
Cellular Localization of Carbonic Anhydrase Nce103p in Candida albicans and Candida parapsilosis
Original language description
Pathogenic yeasts Candida albicans and Candida parapsilosis possess a ß-type carbonic anhydrase Nce103p, which is involved in CO2 hydration and signaling. C. albicans lacking Nce103p cannot survive in low CO2 concentrations, e.g., in atmospheric growth conditions. Candida carbonic anhydrases are orthologous to the Saccharomyces cerevisiae enzyme, which had originally been detected as a substrate of a non-classical export pathway. However, experimental evidence on localization of C. albicans and C. parapsilosis carbonic anhydrases has not been reported to date. Immunogold labeling and electron microscopy used in the present study showed that carbonic anhydrases are localized in the cell wall and plasmatic membrane of both Candida species. This localization was confirmed by Western blot and mass spectrometry analyses of isolated cell wall and plasma membrane fractions. Further analysis of C. albicans and C. parapsilosis subcellular fractions revealed presence of carbonic anhydrases also in the cytosolic and mitochondrial fractions of Candida cells cultivated in shaken liquid cultures, under the atmospheric conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA17-08343S" target="_blank" >GA17-08343S: Study of structures of carbonic anhydrases from pathogenic Candida spp. as a tool for design of potential antimycotics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
—
Volume of the periodical
21
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
8
Pages from-to
850
UT code for WoS article
000522551603004
EID of the result in the Scopus database
2-s2.0-85078852515