The Cwr1 protein kinase localizes to the plasma membrane and mediates resistance to cell wall stress in Candida albicans
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F24%3A00603466" target="_blank" >RIV/68378041:_____/24:00603466 - isvavai.cz</a>
Result on the web
<a href="https://journals.asm.org/doi/10.1128/msphere.00391-24" target="_blank" >https://journals.asm.org/doi/10.1128/msphere.00391-24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/msphere.00391-24" target="_blank" >10.1128/msphere.00391-24</a>
Alternative languages
Result language
angličtina
Original language name
The Cwr1 protein kinase localizes to the plasma membrane and mediates resistance to cell wall stress in Candida albicans
Original language description
The plasma membrane is critical for the virulence of the human fungal pathogen Candida albicans. In addition to functioning as a protective barrier, the plasma membrane plays dynamic roles in a wide range of functions needed for virulence including nutrient uptake, cell wall synthesis, morphogenesis, resistance to stress, and invasive hyphal growth. Screening a collection of C. albicans mutants identified an understudied gene that is important for invasive hyphal growth, which we have termed CWR1 (Cell Wall Regulatory kinase). A mutant strain lacking CWR1 displayed defects in resisting stressful conditions that exacerbate cell wall defects. The Cwr1 protein shows strong similarity to protein kinases, suggesting it plays a regulatory role in coordinating plasma membrane and cell wall functions. A Cwr1–green fluorescent protein (GFP) fusion protein localized to punctate patches associated with the plasma membrane that partially overlapped Membrane Compartment of Can1 (MCC)/eisosome domains. In contrast to the static MCC/eisosome domains, the Cwr1–GFP patches were very dynamic. Truncation mutants lacking C-terminal sequences distal to the protein kinase domain failed to show detectable localization at the plasma membrane. Surprisingly, these mutant strains did not show the defects of a cwr1Δ mutant, suggesting that localization to punctate patches associated with the plasma membrane is not essential for Cwr1 function. Altogether, these data indicate that Cwr1 contributes to the regulation of plasma membrane functions that promote proper morphogenesis and resistance to cell wall stress, both of which are important for C. albicans virulence.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
mSphere
ISSN
2379-5042
e-ISSN
2379-5042
Volume of the periodical
9
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
e0039124
UT code for WoS article
001367259000001
EID of the result in the Scopus database
2-s2.0-85212570607