Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00531479" target="_blank" >RIV/61388963:_____/20:00531479 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/20:00342585
Result on the web
<a href="https://doi.org/10.1038/s41598-020-66593-7" target="_blank" >https://doi.org/10.1038/s41598-020-66593-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-020-66593-7" target="_blank" >10.1038/s41598-020-66593-7</a>
Alternative languages
Result language
angličtina
Original language name
Simultaneous label-free live imaging of cell nucleus and luminescent nanodiamonds
Original language description
In recent years, fluorescent nanodiamond (fND) particles containing nitrogen-vacancy (NV) centers gained recognition as an attractive probe for nanoscale cellular imaging and quantum sensing. For these applications, precise localization of fNDs inside of a living cell is essential. Here we propose such a method by simultaneous detection of the signal from the NV centers and the spectroscopic Raman signal from the cells to visualize the nucleus of living cells. However, we show that the commonly used Raman cell signal from the fingerprint region is not suitable for organelle imaging in this case. Therefore, we develop a method for nucleus visualization exploiting the region-specific shape of C-H stretching mode and further use k-means cluster analysis to chemically distinguish the vicinity of fNDs. Our technique enables, within a single scan, to detect fNDs, distinguish by chemical localization whether they have been internalized into cell and simultaneously visualize cell nucleus without any labeling or cell-fixation. We show for the first time spectral colocalization of unmodified high-pressure high-temperature fND probes with the cell nucleus. Our methodology can be, in principle, extended to any red- and near-infrared-luminescent cell-probes and is fully compatible with quantum sensing measurements in living cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
Jun 17
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
9791
UT code for WoS article
000543956500031
EID of the result in the Scopus database
2-s2.0-85086739076