Capillary electrophoretic profiling of in‐bone tryptic digests of proteins as a potential tool for the detection of inflammatory states in oral surgery
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00532117" target="_blank" >RIV/61388963:_____/20:00532117 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/20:10417285 RIV/60461373:22340/20:43921485
Result on the web
<a href="https://doi.org/10.1002/jssc.202000718" target="_blank" >https://doi.org/10.1002/jssc.202000718</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.202000718" target="_blank" >10.1002/jssc.202000718</a>
Alternative languages
Result language
angličtina
Original language name
Capillary electrophoretic profiling of in‐bone tryptic digests of proteins as a potential tool for the detection of inflammatory states in oral surgery
Original language description
The commonly used histological assessment of pathological states of alveolar bone tissues in oral surgery needs laborious and time‐consuming processing by an experienced histologist. Therefore, a simpler and faster methodology is required in this field. Following this demand, this paper reports a straightforward approach using the tryptic cleavage of proteins directly in bone without its demineralization, followed by the capillary electrophoresis‐ultraviolet detection profiling of the yielded protein digest. Cleavage‐derived peptides were separated by capillary electrophoresis in acidic background electrolytes, pH 2.01–2.54. The best resolution of peptide fragments with the highest peak capacity was achieved in the background electrolyte composed of 55 mM H3PO4, 14 mM tris(hydroxymethyl)aminomethan, pH 2.01. The differences in the obtained capillary electrophoresis‐ultraviolet detection profiles with characteristic patterns for particular bone samples were subsequently discriminated by linear discriminant analysis over principal components. This approach was first verified on porcine bone tissues as model samples, jawbone and calf bone tissues could be discriminated with an accuracy of 100%. Subsequently, the method was capable of differentiating unequivocally between human healthy and inflammatory alveolar bone tissues obtained from oral surgery. This procedure seems to be promising as complement or even an alternative to the traditional histological discrimination between healthy and inflammatory bone tissues in oral surgery.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Separation Science
ISSN
1615-9306
e-ISSN
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Volume of the periodical
43
Issue of the periodical within the volume
20
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
3949-3959
UT code for WoS article
000562972900001
EID of the result in the Scopus database
2-s2.0-85089782415