Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00533050" target="_blank" >RIV/61388963:_____/20:00533050 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/20:00533050 RIV/60461373:22310/20:43921852 RIV/60461373:22330/20:43921852 RIV/00216208:11310/20:10442516

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0144861720307621" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0144861720307621</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbpol.2020.116588" target="_blank" >10.1016/j.carbpol.2020.116588</a>

Result language

angličtina

Original language name

Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Original language description

Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 x 10(4)g mol(-1)) was defined mainly as branched O-2-beta-xylo-(1> 3)-beta-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 x 10(5) and 9.84 x 10(4)g mol(-1)) were complex polysaccharides containing alpha-L-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-alpha production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Carbohydrate Polymers

ISSN

0144-8617

e-ISSN

—

Volume of the periodical

246

Issue of the periodical within the volume

OCT 15

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

116588

UT code for WoS article

000554907700012

EID of the result in the Scopus database

2-s2.0-85086898107