EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00551576" target="_blank" >RIV/61388963:_____/22:00551576 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.15252/embr.202153210" target="_blank" >https://doi.org/10.15252/embr.202153210</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15252/embr.202153210" target="_blank" >10.15252/embr.202153210</a>
Alternative languages
Result language
angličtina
Original language name
EMC is required for biogenesis of Xport-A, an essential chaperone of Rhodopsin-1 and the TRP channel
Original language description
The ER membrane protein complex (EMC) is required for the biogenesis of a subset of tail anchored (TA) and polytopic membrane proteins, including Rhodopsin-1 (Rh1) and the TRP channel. To understand the physiological implications of EMC-dependent membrane protein biogenesis, we perform a bioinformatic identification of Drosophila TA proteins. From 254 predicted TA proteins, screening in larval eye discs identified two proteins that require EMC for their biogenesis: fan and Xport-A. Fan is required for male fertility in Drosophila and we show that EMC is also required for this process. Xport-A is essential for the biogenesis of both Rh1 and TRP, raising the possibility that disruption of Rh1 and TRP biogenesis in EMC mutants is secondary to the Xport-A defect. We show that EMC is required for Xport-A TMD membrane insertion and that EMC-independent Xport-A mutants rescue Rh1 and TRP biogenesis in EMC mutants. Finally, our work also reveals a role for Xport-A in a glycosylation-dependent triage mechanism during Rh1 biogenesis in the endoplasmic reticulum.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Embo Reports
ISSN
1469-221X
e-ISSN
1469-3178
Volume of the periodical
23
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
e53210
UT code for WoS article
000731038800001
EID of the result in the Scopus database
2-s2.0-85121348239