Synthesis of N-acetyl-L-aspartyl-L-glutamic acid, [3H]NAAG

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00559559" target="_blank" >RIV/61388963:_____/22:00559559 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/jlcr.3991" target="_blank" >https://doi.org/10.1002/jlcr.3991</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jlcr.3991" target="_blank" >10.1002/jlcr.3991</a>

Result language
angličtina
Original language name
Synthesis of N-acetyl-L-aspartyl-L-glutamic acid, [3H]NAAG
Original language description
[3H]NAAG,N-acetyl-L-aspartyl-L-glutamic acid, has been widely used as a sub-strate in glutamate carboxypeptidase II (GCPII) reactions, either to determinethe inhibitory constants at 50% inhibition (IC50) of novel compounds or tomeasure GCPII activities in different tissues harvested from various diseasemodels. The importance of [3H]NAAG, combined with its current commercialunavailability, prompted the development of a reliable eight-step synthetic pro-cedure towards [3H2]NAAG starting from commercially available pyrogluta-mate. Pure [3H]NAAG of high molar activity (49.8 Ci/mmol) and desiredstereochemistry was isolated in high radiochemical yield (67 mCi) and radio-chemical purity (>99%). The identity was confirmed by mass spectrometry andco-injection with unlabeled reference.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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