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Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00560854" target="_blank" >RIV/61388963:_____/22:00560854 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1002/smll.202201395" target="_blank" >https://doi.org/10.1002/smll.202201395</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/smll.202201395" target="_blank" >10.1002/smll.202201395</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells

  • Original language description

    Diamond magnetometry can provide new insights on the production of free radicals inside live cells due to its high sensitivity and spatial resolution. However, the measurements often lack intracellular context for the recorded signal. In this paper, the possible use of single-particle tracking and trajectory analysis of fluorescent nanodiamonds (FNDs) to bridge that gap is explored. It starts with simulating a set of different possible scenarios of a particle's movement, reflecting different modes of motion, degrees of confinement, as well as shapes and sizes of that confinement. Then, the insights from the analysis of the simulated trajectories are applied to describe the movement of FNDs in glycerol solutions. It is shown that the measurements are in good agreement with the previously reported findings and that trajectory analysis yields meaningful results, when FNDs are tracked in a simple environment. Then the much more complex situation of FNDs moving inside a live cell is focused. The behavior of the particles after different incubation times is analyzed, and the possible intracellular localization of FNDs is deducted from their trajectories. Finally, this approach is combined with long-term magnetometry methods to obtain maps of the spin relaxation dynamics (or T1) in live cells, as FNDs move through the cytosol.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    21001 - Nano-materials (production and properties)

Result continuities

  • Project

    <a href="/en/project/EF16_026%2F0008382" target="_blank" >EF16_026/0008382: Carbon allotropes with rationalized nanointerfaces and nanolinks for environmental and biomedical applications</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Small

  • ISSN

    1613-6810

  • e-ISSN

    1613-6829

  • Volume of the periodical

    18

  • Issue of the periodical within the volume

    39

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    17

  • Pages from-to

    2201395

  • UT code for WoS article

    000847207900001

  • EID of the result in the Scopus database

    2-s2.0-85130576223