Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00560854" target="_blank" >RIV/61388963:_____/22:00560854 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/smll.202201395" target="_blank" >https://doi.org/10.1002/smll.202201395</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/smll.202201395" target="_blank" >10.1002/smll.202201395</a>
Alternative languages
Result language
angličtina
Original language name
Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells
Original language description
Diamond magnetometry can provide new insights on the production of free radicals inside live cells due to its high sensitivity and spatial resolution. However, the measurements often lack intracellular context for the recorded signal. In this paper, the possible use of single-particle tracking and trajectory analysis of fluorescent nanodiamonds (FNDs) to bridge that gap is explored. It starts with simulating a set of different possible scenarios of a particle's movement, reflecting different modes of motion, degrees of confinement, as well as shapes and sizes of that confinement. Then, the insights from the analysis of the simulated trajectories are applied to describe the movement of FNDs in glycerol solutions. It is shown that the measurements are in good agreement with the previously reported findings and that trajectory analysis yields meaningful results, when FNDs are tracked in a simple environment. Then the much more complex situation of FNDs moving inside a live cell is focused. The behavior of the particles after different incubation times is analyzed, and the possible intracellular localization of FNDs is deducted from their trajectories. Finally, this approach is combined with long-term magnetometry methods to obtain maps of the spin relaxation dynamics (or T1) in live cells, as FNDs move through the cytosol.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21001 - Nano-materials (production and properties)
Result continuities
Project
<a href="/en/project/EF16_026%2F0008382" target="_blank" >EF16_026/0008382: Carbon allotropes with rationalized nanointerfaces and nanolinks for environmental and biomedical applications</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Small
ISSN
1613-6810
e-ISSN
1613-6829
Volume of the periodical
18
Issue of the periodical within the volume
39
Country of publishing house
DE - GERMANY
Number of pages
17
Pages from-to
2201395
UT code for WoS article
000847207900001
EID of the result in the Scopus database
2-s2.0-85130576223